Product Name :
Phospho-p95/NBS1 (S343) Recombinant Rabbit Monoclonal Antibody [SY0215]
Predicted band size :
85 kDa
Observed band size :
95 kDa
Synonyms:
AT V1 antibody AT V2 antibody ATV antibody Cell cycle regulatory protein p95 antibody FLJ10155 antibody MGC87362 antibody Nbn antibody NBN_HUMAN antibody NBS 1 antibody NBS antibody 展开
Function :
Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. These phenotypes are similar to those of ataxia telangiectasia mutated (ATM) disease, where there is a deficiency in a protein kinase that is activated by DNA damage, indicating that the NBS1 (Nibrin) and ATM proteins may participate in common pathways. Nibrin is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. The phosphorylation of Nibrin requires catalytically active ATM. ATM physically interacts with and phosphorylates Nibrin on Serine 343 both in vitro and in vivo. Serine 343 is phosphorylated in vitro by ATM and the modification of this residue in vivo is essential for the cellular response to DNA damage.Price of N-Methyl-L-valine This response includes S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation.6-Bromochroman-4-amine Chemscene
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser343 of Human p95 / NBS1 aa 321-370 / 754.
Species reactivity:
Human, Mouse
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, IP
Coupling :
unconjugated
Clone number:
SY0215
RRID:
AB_3069769
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.PMID:33432822 Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 85 kDa
Positive control :
HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, Jurkat treated with 25μM Etoposide for 5 hours whole cell lysate, PC-3M, mouse testis tissue.