Product Name :
Phospho-Cdc6 (S54) Recombinant Rabbit Monoclonal Antibody [SD08-47]
Predicted band size :
63 kDa
Observed band size :
63 kDa
Synonyms:
Cdc 18L antibody Cdc 6 antibody CDC18 (cell division cycle 18, S.pombe, homolog) like antibody CDC18 (S.pombe) antibody Cdc18 related protein antibody CDC18(S.pombe) antibody Cdc18-related protein antibody Cdc18L antibody cdc6 antibody CDC6 cell division cycle 6 homolog antibody 展开
Function :
Cdc6, or cell division cycle 6, is a protein in eukaryotic cells. CDC6 is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). CDC6 assembles after ORC in an ATP dependent manner and is required for loading MCM proteins onto the DNA. It is thought that the CDC6-Cdt1 complex uses ATP hydrolysis to thread DNA through the central hole of the MCM doughnut. Mutations in the binding motif of CDC6 strongly suggest that ATP binding and hydrolysis is essential for its function.13315-17-8 supplier The minimal requirement for DNA binding has been mapped within its 47-amino acid sequence.2,4,6-Triformylphloroglucinol Order Furthermore, Cdc6 indirectly inhibits activation of the p34cdc2/CDC28 M phase kinase, thus nuclear division is suppressed.PMID:33487045
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser54 of Human Cdc6 aa 41-90 / 560.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P
Coupling :
unconjugated
Clone number:
SD08-47
RRID:
AB_3073461
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 63 kDa
Positive control :
Jurkat cell lysates, HeLa whole cell lysate, HeLa treated with 3mM Hydroxyurea for 20 hours whole cell lysate, Hela, HepG2, A431, mouse spleen tissue, rat spleen tissue.